Protein_Domain

Part:BBa_K5466012:Design

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-23)


CadC intracellular domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild type transmembrane region was sustituyed by an artificial Leu (16) transmembrane region, to improve the receptor signal-to-noise ratio by decreasing its background noise. Without stop codon for fusion with LBD.

Optimized to E. coli

Source

Chang, H., Mayonove, P., Zavala, A., De Visch, A., Minard, P., Cohen-Gonsaud, M., & Bonnet, J. (2017). A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria. ACS Synthetic Biology, 7(1), 166-175. https://doi.org/10.1021/acssynbio.7b00266